Double Emulsion Picoreactors for High-Throughput Single-Cell Encapsulation and Phenotyping via FACS
نویسندگان
چکیده
منابع مشابه
High throughput single-cell and multiple-cell micro-encapsulation.
UNLABELLED Microfluidic encapsulation methods have been previously utilized to capture cells in picoliter-scale aqueous, monodisperse drops, providing confinement from a bulk fluid environment with applications in high throughput screening, cytometry, and mass spectrometry. We describe a method to not only encapsulate single cells, but to repeatedly capture a set number of cells (here we demons...
متن کاملHigh Throughput Single Cancer Cell Encapsulation and Self Sorting for Protease Assay by Using Jetting Microfluidics
In this work, a droplet microfluidic device integrated with droplet sorting function was presented for single cancer cell encapsulations and protease activity measurements. Individual cells were encapsulated into aqueous droplets at flow focusing junction under jetting conditions. Droplets with cells encapsulated inside are larger than other empty droplets, enabling effective droplet sorting th...
متن کاملMicrodroplet Emulsion Generator Arrays for High-throughput Single Cell Genetic Variation Analysis
Single cell resolution technology enables the investigation of rare biological events masked by the ensemble average. We have developed a high-throughput microdroplet emulsion generator array (MEGA) as a core platform for massively parallel emulsion PCR-based detection of single cells with a frequency lower than 10 [1, 2]. We have recently used this platform to detect E. coli O157 in a backgrou...
متن کاملHigh-throughput hyperdimensional vertebrate phenotyping
Most gene mutations and biologically active molecules cause complex responses in animals that cannot be predicted by cell culture models. Yet animal studies remain too slow and their analyses are often limited to only a few readouts. Here we demonstrate high-throughput optical projection tomography with micrometre resolution and hyperdimensional screening of entire vertebrates in tens of second...
متن کاملHigh-throughput phenotyping technology for maize roots
* Corresponding author. E-mail address: [email protected] (T.E. Gr 1537-5110/$ e see front matter a 2011 IAgrE doi:10.1016/j.biosystemseng.2011.06.004 This paper describes the development of high-throughput measurement techniques allowing acquisition of phenotypical data describing maize roots. One of a maize root’s traits is the level of complexity, which was expressed in a Fractal Dimension ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Analytical Chemistry
سال: 2020
ISSN: 0003-2700,1520-6882
DOI: 10.1021/acs.analchem.0c02499